MIL-OSI USA: Detecting Hydrothermal Vents and Collecting Environmental DNA: Investigating the Water Column in the Aleutian Arc

Source: US Geological Survey

Exploring the deep ocean along the Aleutian Arc requires a suite of advanced tools to investigate the intricate connections between marine ecosystems and their dynamic environment. One of the most valuable instruments aboard any deep-sea exploration cruise is the CTD, which stands for Conductivity, Temperature, and Depth. This essential oceanographic tool provides detailed information on the physical and chemical structure of the water column—from detecting hydrothermal vents to collecting environmental DNA (eDNA), genetic material that organisms have shed into the surrounding water.

A USGS scientist prepares the Niskin bottles on the CTD before deployment. Image courtesy of The Aleutian Arc: Integrated Exploration of Biodiversity at Priority Benthic Habitats (USGS/BOEM/NOAA/ONR). Photographer: Art Howard. 

Deep-sea benthic communities found along the Aleutian Arc are sensitive to subtle environmental gradients. A CTD profile helps pinpoint areas where these communities might thrive by revealing thermoclines; oxygen minimum zone; and variations in salinity, turbidity, and chemical composition. In regions where hydrothermal activity is suspected, CTD profiles often reveal sharp temperature spikes, plumes of turbidity caused by mineral precipitates, and elevated concentrations of metals or other chemical tracers.

Each CTD is equipped with an array of sensors and a rosette of Niskin bottles, which collect discrete water samples at targeted depths. On this expedition, these samples are analyzed for geochemical signatures and used in eDNA analyses, enabling researchers to detect “who is there,” including cryptic species that are difficult to find. This integration of physical, chemical, and biological data help establish connections between water column properties and the distribution of deep-sea life.

Water collected by Niskin bottles can be analyzed for environmental DNA studies, which helps USGS researchers detect genetic material left behind by organisms in the surrounding water. Image courtesy of The Aleutian Arc: Integrated Exploration of Biodiversity at Priority Benthic Habitats (USGS/BOEM/NOAA/ONR). Photographer: Art Howard. 

Combined with data collected during Alvin submersible dives and seafloor mapping, CTD data are crucial in guiding site selection for biological sampling and in interpreting the conditions shaping habitats along the Aleutian Arc. This integrated approach supports both scientific discovery and the informed stewardship of deep-sea habitats and resources.

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